anti-CD178 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD178; anti-CD178 antibody;
The CD178 faslg (Catalog #MBS213807) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD178 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*.Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat; Application Note: Results maybe enhanced using membrane permeabilisation. MyBioSource recommends the use of LeucopermTM prior to staining. Researchers should empirically determine the suitability of the CD178 faslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD178 faslg product has the following accession number(s) (GI #4557329) (NCBI Accession #NP_000630.1) (Uniprot Accession #P48023). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD178:RPE with the following immunoassay(s):
Testing Data (Staining of permeabilised Jurkat cells with Mouse anti Human CD178 (MBS212780))
Testing Data (Staining of CD178 transfected CHO cells with Mouse anti Human CD178: Alexa Fluor 647 (MBS212780A647))
Testing Data (Staining of permeabilised Jurkat cells with Mouse anti Human CD178 (MBS212781))
Testing Data (Staining of permeabilised Jurkat cells with Mouse anti Human CD178:Alexa Fluor 488 (MBS212780A488))
Testing Data (Staining of permeabilised Jurkat cells with Mouse anti Human CD178:RPE)
Testing Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MBS212780) as a capture reagent and biotinylated Mouse anti Human CD178 (MBS212778) as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader. Plasma (orange) sample is displayed at 1:2 dilution.)
Mouse anti Human CD178 antibody, clone 14C2 recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40kDa single pass type-II transmembrane glycoprotein bearing a single intracellular FasL domain and member of the tumor necrosis factor family. CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995). Mouse anti human CD178 antibody, clone 14C2 reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).