anti-CD172a antibody product blog
Tags: Antibody; Monoclonal Antibody; CD172a; anti-CD172a antibody;
The CD172a sirpa (Catalog #MBS213862) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD172a can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD172a sirpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD172a sirpa product has the following accession number(s) (GI #31543529) (NCBI Accession #NP_037148.2) (Uniprot Accession #P97710). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD172a:RPE with the following immunoassay(s):
Testing Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE)
Testing Data (Published clone specific image: Flow cytometric analysis of ED7 and ED9 expression of AM following magnetic bead separation. AM of 3 days NO2-exposed rats were separated due to their expression of the cell surface molecule ED7 using magnetic bead separation. Susbsequently, ED7 (left) and ED9 (right) expression was analyzed in unseparated AM (A), ED7-positive AM (B), and ED7-negative AM (C). Numbers right of each histogram represent the mean fluorescence of the respective cell population. The figure clearly demonstrates that ED7-positive AM show a lower ED9 expression compared to ED7-negative AM. Shown is a representative data set of more than twenty animals.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)
Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power)
Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9, red in An and Mouse anti Rat CD4, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9, red in An and Mouse anti Rat CD4, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power)
Testing Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)
Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRP&alpha, -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid %#126;56 kDa single pass typew 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons(UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OCX-41 (Adams et al. 1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al. 2002).