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anti-CD16/CD32 antibody product blog

Posted on 2014-09-20 08:52:53 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD16/CD32; anti-CD16/CD32 antibody;
The CD16/CD32 fcgr2 (Catalog #MBS211233) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD16/CD32 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Functional Assays (FN).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD16/CD32 fcgr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD16/CD32 fcgr2 product has the following accession number(s) (GI #6857783) (NCBI Accession #NP_034317.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD16/CD32:Low Endotoxin with the following immunoassay(s):
Testing Data (Staining of mouse peripheral blood lymphocytes Rat anti Mouse CD16/32: Alexa Fluor 488 (MBS212682A488))
Testing Data CD16/CD32.

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD16/32 : RPE (MBS213784))
Testing Data CD16/CD32.

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD16/32: FITC (MBS210943))
Testing Data CD16/CD32.

Testing Data (Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD16/32: Biotin (MBS210447))
Testing Data CD16/CD32.

Rat anti Mouse CD16/CD32 antibody, clone FCR4G8 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.

Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: PU5 1.8 IOE7 Balb/c mouse cell line.
Fusion Partners: Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD16/CD32 are readily searchable from our website. Different antibodies against the same target such as CD16/CD32 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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