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anti-CD14 antibody product blog

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Posted on 2014-11-06 12:50:41 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD14; anti-CD14 antibody;
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The CD14 cd14 (Catalog #MBS215094) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD14 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD14 cd14 product has the following accession number(s) (GI #4557417) (NCBI Accession #NP_000582.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD14:RPE with the following immunoassay(s):
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Pacific Blue (MBS212217PB))
Testing Data CD14.

Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 647 (MBS212217P647))
Testing Data CD14.

Testing Data (Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Testing Data CD14.

Testing Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Testing Data CD14.

Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin (MBS219364))
Testing Data CD14.

Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14 (MBS212218))
Testing Data CD14.

Testing Data (Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Testing Data CD14.

Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14 (MBS211203))
Testing Data CD14.

Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 750 (MBS212217P750))
Testing Data CD14.

Testing Data (Staining of human peripheral blood monocytes probed with Mouse anti Human CD14:FITC (MBS210731))
Testing Data CD14.

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