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anti-CD120a antibody product blog

Posted on 2014-01-31 15:20:09 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD120a; anti-CD120a antibody;
The CD120a tnfrsf1a (Catalog #MBS214946) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD120a can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD120a tnfrsf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD120a tnfrsf1a product has the following accession number(s) (GI #4507575) (NCBI Accession #NP_001056.1) (Uniprot Accession #P19438). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD120a:FITC with the following immunoassay(s):
Testing Data (Staining of human peripheral blood granulocytes with Mouse anti human CD120a: Alexa Fluor 647 (MBS212106A647))
Testing Data CD120a.

Mouse anti Human CD120a antibody, clone H398 recognizes an extracellular domain of the 55kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75kDa TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes. Mouse anti Human CD120a antibody, clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application. Mouse anti Human CD120a antibody, clone H398 inhibits the biological activity of both natural and recombinant human TNFalpha and TNFbeta (Thoma et al. 1990and Dri et al. 1999). Mouse anti Human CD120a antibody, clone H398 is routinely tested in flow cytometry on human peripheral blood monocytes.

Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD120a are readily searchable from our website. Different antibodies against the same target such as CD120a may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CD120a also interacts with the following gene(s): BIRC2, FADD, IKBKB, LTA, RIPK1, TNF, TRADD, TRAF1, TRAF2.
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