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anti-CD11b antibody product blog

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Posted on 2013-09-08 23:41:32 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD11b; anti-CD11b antibody;
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The CD11b n/a (Catalog #MBS520037) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD11b, Biotin (Clone M1/70.15) (rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD11b can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD11b n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD11b n/a product has the following accession number(s) (GI #1616950) (NCBI Accession #AAB16869.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Mouse CD11b, Biotin (Clone M1/70.15) (rat IgG2b) with the following immunoassay(s):
Testing Data
Testing Data CD11b.

anti-mouse CD11b (Mac-1; Ly 40) monoclonal antibody is specific for the 170 kDa a subunit of Mac-1 which mediates adhesion to ICAM-1 (CD54) and C3bi. Mac-1 is expressed on granuloytes, macrophages, natural killer cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 is up-regulated on neutrophils after activation. This particular clone blocks cell adherence and C3bi binding but does not block cell medited lysis (1,2,3,4,5,6). Applications include flow cytometry, in vitro and in vivo blocking, immunohistochemistry for both paraffin embedded and acetone-fixed frozen sections 1-20 ug/ml (#7). Also used for immunoprecipitation and western blotting.

Immunogen: C57BL/10 spleen cell enriched for T lymphocytes
Donor: DA rat spleen
Fusion Partner: NS-1. Presentation: 100 ug or 300 ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD11b are readily searchable from our website. Different antibodies against the same target such as CD11b may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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