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anti-CD11b antibody product blog

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Posted on 2013-11-15 00:01:10 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD11b; anti-CD11b antibody;
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The CD11b itgam (Catalog #MBS219260) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD11b can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100;
Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details. Researchers should empirically determine the suitability of the CD11b itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD11b itgam product has the following accession number(s) (GI #124956) (NCBI Accession #P05555.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD11b:Low Endotoxin with the following immunoassay(s):
Testing Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power)
Testing Data CD11b.

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647 )
Testing Data CD11b.

Testing Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power)
Testing Data CD11b.

Testing Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC (MBS211086))
Testing Data CD11b.

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE (MBS213875))
Testing Data CD11b.

Testing Data (Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells\' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)
Testing Data CD11b.

Testing Data (Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE (MBS213874))
Testing Data CD11b.

Testing Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488 )
Testing Data CD11b.

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b (MBS213144))
Testing Data CD11b.

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b (MBS213143))
Testing Data CD11b.

Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: T cell enriched splenocytes from B10 mice
Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse.
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