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anti-CD11a antibody product blog

Posted on 2013-12-19 05:48:07 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD11a; anti-CD11a antibody;
The CD11a n/a (Catalog #MBS213156) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD11a can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD11a n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD11a n/a product has the following accession number(s) (GI #77404269) (NCBI Accession #NP_001029170.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD11a with the following immunoassay(s):
Testing Data (Staining of rat spleen cells with Mouse anti Rat CD11a:Alexa Fluor 647 (MBS213155A647))
Testing Data CD11a.

Testing Data (Staining of rat splenocytes with Mouse anti Rat CD11a: Azide Free (MBS210272))
Testing Data CD11a.

Testing Data (Staining of rat spleen cells with Mouse anti Rat CD11a:Alexa Fluor 488 (MBS213155A488))
Testing Data CD11a.

Mouse anti Rat CD11a antibody, clone WT.1 reacts with rat CD11a, a glycoprotein of 160-170kD which is associated with CD18. CD11a is one of the leucocyte integrins (b2 integrin) which plays an important role in leucocyte physiology. Functionally, this monoclonal inhibits homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.

Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Splenic PHA Blasts
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the PAI mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Rat. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD11a are readily searchable from our website. Different antibodies against the same target such as CD11a may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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