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anti-CD105 antibody product blog

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Posted on 2014-01-31 12:10:14 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD105; anti-CD105 antibody; CD105/Endoglin, PE conjugated;
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The CD105 eng (Catalog #MBS690852) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD105/Endoglin reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD105/Endoglin, PE conjugated can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
The suggested use of the antibody is <=0.5ug in 100 ul volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application. Researchers should empirically determine the suitability of the CD105 eng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD105 eng product has the following accession number(s) (GI #226437647) (NCBI Accession #NP_031958.2) (Uniprot Accession #Q63961). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rat Anti-Mouse CD105/Endoglin with the following immunoassay(s):
Testing Data
Testing Data CD105.

Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 70-75 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human Endoglin, the S-Endoglin and L-Endoglin that differ in the length of their cytoplasmic teails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondroytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pre-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse Endoglin share approximately 70% and 97% amino acid sequence identity in their extracellular and intracellular domains, respectively. Endoglin binds TGF-beta1 and TGF-beta3 but not TGF-beta2 efficiently by associating with TGF-beta tyme II receptor (TbetaRII). Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.

Immunogen: murine stromal cell line
Conjugation: PE
Preparation: Monoclonal antibodies were produced by immunizing rats with inflamed mouse skin. Buffer/Stabilizer: PBS; 1% BSA
Preservative: 0,02% sodium azide
Warning: Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD105 are readily searchable from our website. Different antibodies against the same target such as CD105 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Bone Marrow, Brain, Connective Tissue, Eye, Heart, Liver, Lung, Muscle, Skin tissues are correlated with this protein. The following patways have been known to be associated with this gene. Atherosclerosis, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Hypertension, Inflammation, Liver Diseases, Necrosis, Neoplasms, Nervous System Diseases are some of the diseases may be linked to Rat Anti-Mouse CD105/Endoglin.
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