anti-CCNE1 antibody product blog
Tags: Antibody; CCNE1; anti-CCNE1 antibody; CCNE1-T395;
The CCNE1 ccne1 (Catalog #MBS125398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CCNE1-T395 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CCNE1-T395 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100. Researchers should empirically determine the suitability of the CCNE1 ccne1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCNE1 ccne1 product has the following accession number(s) (GI #3041657) (NCBI Accession #P24864.2) (Uniprot Accession #P24864). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Phospho-CCNE1-T395 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using Phospho-CCNE1-T395 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cells using Phospho-CCNE1-T395 antibody. Blue: DAPI for nuclear staining.)
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
Species: Human
Route: Synthetic Peptide. Immunogen: A phospho specific peptide corresponding to residues surrounding T395 of human CCNE1
Calculated Molecular Weight: 47kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CCNE1 are readily searchable from our website. Different antibodies against the same target such as CCNE1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.