|The CCNB1 ccnb1 (Catalog #MBS850319) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CCNB1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CCNB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA).
WB: 1/500 - 1/2000, IF: 1/200 - 1/1000, FC: 1/200-1/400, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Researchers should empirically determine the suitability of the CCNB1 ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCNB1 ccnb1 product has the following accession number(s) (GI #14327896) (NCBI Accession #NP_114172.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CCNB1 Antibody with the following immunoassay(s):
Testing Data (Figure 1: Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.)
Testing Data #2 (Figure 2: Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Testing Data #3 (Figure 3: Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).)
Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues.
Immunogen: Purified recombinant fragment of human CCNB1 expressed in E. Coli.
Species: Mouse IgG1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CCNB1 are readily searchable from our website. Different antibodies against the same target such as CCNB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CCNB1 also interacts with the following gene(s): ANAPC11, ANAPC7, CDC16, CDC20, CDC23, CDC27, CDK1. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Hepatocellular, Carcinoma, Squamous Cell, Disease Models, Animal, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Neoplasms, Experimental, Prostatic Neoplasms are some of the diseases may be linked to CCNB1 Antibody.