anti-CBX3 antibody product blog
Tags: Antibody; Polyclonal Antibody; CBX3; anti-CBX3 antibody; CBX3/HP1 Gamma;
The CBX3 cbx3 (Catalog #MBS420248) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CBX3/HP1 Gamma Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s CBX3/HP1 Gamma can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.
Western Blot: Approx.20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1. 0 ug/ml. Researchers should empirically determine the suitability of the CBX3 cbx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CBX3 cbx3 product has the following accession number(s) (GI #15082258) (NCBI Accession #NP_009207.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
EAFLNSQKAG KEKD.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-CBX3/HP1 Gamma Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) ((2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunogen: Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2; NP_057671.2.
Epitope: Internal region. Note: No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CBX3 are readily searchable from our website. Different antibodies against the same target such as CBX3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CBX3 also interacts with the following gene(s): CBX1, CBX5, DNMT3B, EHMT2, TRIM28. The following patways have been known to be associated with this gene. Blood, Brain, Embryonic Tissue, Kidney, Liver, Lung, Muscle, Ovary, Testis, Vascular tissues are correlated with this protein.