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anti-CBL antibody product blog

Posted on 2013-08-28 21:55:57 by mybiosource_staff
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Tags: Antibody; anti-CBL antibody; CBL; Polyclonal Antibody;
The CBL cbl (Catalog #MBS150368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cbl Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Cbl can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF).
Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunoflourescence starting at 20 mug/mL. Researchers should empirically determine the suitability of the CBL cbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CBL cbl product has the following accession number(s) (GI #115855) (NCBI Accession #P22681.1) (Uniprot Accession #P22681). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Cbl Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.)
Western Blot (WB) CBL.

Immunofluorescence (IF) (Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 μg/mL.)
Immunofluorescence (IF) CBL.

Cbl Antibody: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.

Conjugate: Unconjugated
Immunogen: Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl.
Buffer: Cbl Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CBL are readily searchable from our website. Different antibodies against the same target such as CBL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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