anti-Cardiotin antibody product blog
Tags: Antibody; Monoclonal Antibody; Cardiotin; anti-Cardiotin antibody;
The Cardiotin n/a (Catalog #MBS570050) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Cardiotin reacts with Human, Swine and may cross-react with other species as described in the data sheet. MyBioSource\'s Cardiotin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Western Blot (WB).SR-3 is useful for immunohistochemistry on frozen sections and immunoblotting. In immunoblotting assays SR-3 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting appliCations. Researchers should empirically determine the suitability of the Cardiotin n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Cardiotin is a high molecular weight protein complex (300 kDa) loCated in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuRation. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to Human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Source Note: SR-3 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with the 100 kDa cardiotin subunit. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Cardiotin are readily searchable from our website. Different antibodies against the same target such as Cardiotin may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.