anti-CANX antibody product blog
Tags: Antibody; Calnexin; Polyclonal Antibody; CANX; anti-CANX antibody;
The CANX canx (Catalog #MBS618627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calnexin reacts with Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s Calnexin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC).Western Blot (Colorimetric) (1): 1:2,000, Immunoprecipitation (1): 1:100, Immunocytochemistry (3): 1:200, Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the CANX canx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CANX canx product has the following accession number(s) (GI #186523) (Uniprot Accession #P27824). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Calnexin with the following immunoassay(s):
Western Blot (WB) (Western blot analysis: Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of human placenta tissue stained with MBS618627 at 5ug/ml.)
Calnexin (CNX), an abundant ~90kD molecular chaperone, is an unglycosylated resident ER transmembrane protein. In mammalian cells, calnexin, together with calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans (4). Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Calnexin associates with numerous oligomeric protein complexes within the ER, including beta1 and alpha6 integrins (5), major histocompatibility class I (6, 7) and class II molecules (8), the antigen receptors expressed on T and B lymphocytes (7), human thyroperoxidase (hTPO) (9), and acetylcholine receptor (10). Recent data also suggest that calnexin might be responsible for the prolonged retention of pro-alpha6 integrin within ER compartment (11). Furthermore, calnexin has been demonstrated to function as a bonafide molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins (12).
Immunogen: A 19 residue synthetic peptide DTSAPTSPKVTYKAPVPSG based on the canine calnexin (residues 50-68) (2) and the peptide coupled to KLH. Positive Control: Heat Shocked HeLa Cell Lysate
. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CANX are readily searchable from our website. Different antibodies against the same target such as CANX may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.