anti-CanA-14F antibody product blog
Tags: Antibody; Polyclonal Antibody; Calcineurin A, pan; anti-CanA-14F antibody; CanA-14F;
The CanA-14F cana-14f (Catalog #MBS616230) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Calcineurin A, pan can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)Other applications have not been tested.
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (IC): 1:50
Flow Cytometry: 1:25
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CanA-14F cana-14f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CanA-14F cana-14f product has the following accession number(s) (GI #56311437) (NCBI Accession #NP_727986.2) (Uniprot Accession #Q9VXF1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Calcineurin A, pan (Protein Phosphatase 2B,PP2B, CnA) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of lysates from MCF7, NIH/3T3, C6 and COS cells, using MBS616230)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of NIH/3T3 cells, using MBS616230(blue) compared to a nonspecific negative control antibody (red))
Immunofluorescence (IF) (Confocal immunofluorescent analysis of Hela cells, using MBS616230exhibiting cytoplasmic localization (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin An is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin An and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin An and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding.
Immunogen: Synthetic peptide corresponding to the C-terminus of human Calcineurin A (alpha isoform). Species sequence homology: chicken, bovine, xenopus and porcine (100%). Cross Reactivity: Mouse, rat, monkey.