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anti-CAMK II ALPHA antibody product blog

Posted on 2014-05-29 21:17:23 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CAMK II ALPHA; anti-CAMK II ALPHA antibody;
The CAMK II ALPHA camk2a (Catalog #MBS224102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI CAMK II ALPHA reacts with Pig (Expected from Sequence), Rabbit (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence) and may cross-react with other species as described in the data sheet. MyBioSource\'s CAMK II ALPHA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB).
ELISA: Minimum Dilution: 1:1000; Maximum Dilution: 1:3000;
Immunohistology - Paraffin: Minimum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1:4000; Maximum Dilution: 1:20000. Researchers should empirically determine the suitability of the CAMK II ALPHA camk2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CAMK II ALPHA camk2a product has the following accession number(s) (GI #25952118) (NCBI Accession #NP_741960.1) (Uniprot Accession #Q9UQM7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI CAMK II ALPHA with the following immunoassay(s):
Testing Data #1 (Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human CAMK II alpha (MBS224102))
Testing Data #1 CAMK II ALPHA.

CaMKII comprises of multiple isoforms that are derived from four closely related gene products; alpha, beta, gamma and delta, that have distinct but overlapping expression patterns. MBS224102 detects Human calcium/calmodulin-dependent protein kinase II alpha (CaMK II alpha), a serine/threonine kinase that is activated by calcium ions (Ca2+) and the Ca2+/calmodulin complex (CaM). CaMK II is expressed in glutamatergic excitatory neurones and constitutes the major postsynaptic density protein. It is important in the regulation of synaptic transmission, with possible roles in learning and memory.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Immunogen: Synthetic peptide sequence corresponding to amino acids 6-23 of human CaMK II A - conjugated to KLH.
Histology Positive Control Tissue: Human brain (cortex)
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human CaMK II alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CAMK II ALPHA are readily searchable from our website. Different antibodies against the same target such as CAMK II ALPHA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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