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anti-C4d antibody product blog

Posted on 2015-03-26 04:37:27 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; C4d; anti-C4d antibody;
The C4d c4b (Catalog #MBS300984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human C4d Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s C4d can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin.
Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Tonsil. Researchers should empirically determine the suitability of the C4d c4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The C4d c4b product has the following accession number(s) (GI #341916194) (NCBI Accession #XP_003403643.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Anti-Human C4d Polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Human tonsil stained with anti-C4d antibody)
Immunohistochemistry (IHC) C4d.

C4 plays a central role in the activation of the classical pathway of the complement system. Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

Immunogen: Synthetic peptide derived from C-terminus of human C4d.
Control Tissue: Kidney, Tonsil
Cellular Localization: Secreted Protein
Entrez Gene Code: 721
Pathway: Complement. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing C4d are readily searchable from our website. Different antibodies against the same target such as C4d may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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