anti-C3a antibody product blog
Tags: Antibody; C3A; Monoclonal Antibody; anti-C3a antibody; C3a RECEPTOR;
The C3a c3ar1 (Catalog #MBS212809) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s C3a RECEPTOR can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20. Researchers should empirically determine the suitability of the C3a c3ar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The C3a c3ar1 product has the following accession number(s) (GI #4757888) (NCBI Accession #NP_004045.1) (Uniprot Accession #Q16581). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN C3a RECEPTOR with the following immunoassay(s):
Testing Data (Published customer image Mouse anti Human C3a receptor antibody, clone hC3aRZ1 used for flow cytometry.Image captionExpression of C3aR1 and C5aR on collagen- and convulxin-activated platelets. Washed platelets were activated for 10 min with 2.5 ug/mL collagen, 500 ng/mL convulxin or saline and further incubated for 30 min at RT and analyzed for C3aR1 and C5aR expression by flow cytometry. A: representative overlays of fluorescence histograms obtained for each condition using an alexa fluor 488 anti-C3aR1 (solid line), a FITC anti-CD88 (dashed line) or a FITC control IgG (dotted line). B: Mean fluorescence intensities +/- SEM of 3 to 9 experiments for each condition.From: Martel C, Cointe S, Maurice P, Matar S, Ghitescu M, et al. (2011) Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets. PLoS ONE 6(4): e18812.)
Mouse anti Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a 55kD cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes. Expression levels of C3aR on human cells varies significantly between donors.
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Recombinant human C3aR protein
Fusion Partners: Cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human.