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anti-BTK antibody product blog

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Posted on 2014-11-16 14:02:06 by mybiosource_staff
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Tags: Antibody; anti-BTK antibody; BTK; Monoclonal Antibody;
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The BTK btk (Catalog #MBS831917) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BTK antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BTK can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB).
FC: 1:100
IF: 1:100
IHC: 1:50
WB: 1:2000. Researchers should empirically determine the suitability of the BTK btk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The BTK btk product has the following accession number(s) (GI #4557377) (NCBI Accession #NP_000052) (Uniprot Accession #Q06187). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BTK antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BTK protein (Right) or empty vector (Left) detected with BTK antibody)
Western Blot (WB) BTK.

Immunohistochemistry (IHC) (Immunohistochemical analysis of BTK protein in paraffin embedded Human liver tissue using BTK antibody)
Immunohistochemistry (IHC) BTK.

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BTK protein using BTK antibody)
Immunofluorescence (IF) BTK.

Mouse monoclonal BTK antibody.

Biological Significance: BTK plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Immunogen: BTK antibody was raised in mouse using a full length recombinant protein of human BTK (NP_000052) produced in HEK293T cells, as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BTK are readily searchable from our website. Different antibodies against the same target such as BTK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Agammaglobulinemia, Atherosclerosis, Bruton type agammaglobulinemia, Cardiovascular Diseases, Chromosome Aberrations, Disease Models, Animal, Inflammation, Kidney Diseases, Necrosis, Nervous System Diseases are some of the diseases may be linked to BTK antibody. Blood, Bone, Bone Marrow, Heart, Liver, Lung, Lymph, Lymph Node, Skin, Spleen tissues are correlated with this protein.
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