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anti-BrdU antibody product blog

Posted on 2014-11-28 09:53:14 by mybiosource_staff
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Tags: Antibody; BRDU; Monoclonal Antibody; anti-BrdU antibody;
The BrdU n/a (Catalog #MBS212833) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s BrdU can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunohistology Paraffin*.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/100; Application Note: See recommended protocol below.
Immunohistology - Paraffin: Application Note: See recommended protocol below. Researchers should empirically determine the suitability of the BrdU n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI BrdU with the following immunoassay(s):
Testing Data (Detection of BrdU incorporation into pulsed-labelled ACNC12 cells using Mouse anti-BrdU antibody (clone Bu20a, MBS212833))
Testing Data BrdU.

Mouse anti BrdU antibody, clone Bu20a recognizes the thymidine analogue bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle. The BU20a antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Chemical. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BrdU are readily searchable from our website. Different antibodies against the same target such as BrdU may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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