|The Human BP180-Ab n/a (Catalog #MBS702977) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702977 ELISA Kit recognizes Human (General) BP180-Ab.
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for complete product instructions).
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with BP180 antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-human IgG and incubate again. Substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. Calculate the valence of anti-BP180-Ab in the samples.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human anti-Bullous Pemphigoid 180 antibody, anti-BP180-Ab ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.