anti-BMP-2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-BMP-2 antibody; BMP-2;
The BMP-2 bmp2 (Catalog #MBS220225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s BMP-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).ELISA: This biotinylated human BMP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human BMP-2 antibody as the capture reagent.
Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
ELISA: Minimum Dilution: 0.25 ug/ml; Maximum Dilution: 1.0 ug/ml
Western Blotting: Minimum Dilution: 0.1 ug/ml; Maximum Dilution: 0.2 ug/ml. Researchers should empirically determine the suitability of the BMP-2 bmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BMP-2 bmp2 product has the following accession number(s) (GI #4557369) (NCBI Accession #NP_001191.1) (Uniprot Accession #P12643). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI HUMAN BMP-2:Biotin with the following immunoassay(s):
Testing Data #1 (Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Testing Data #2 (Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Testing Data #3 (U-2 osteosarcoma cells stained with Rabbit anti Human BMP-2 (MBS221841) green, tubulin, red and nuclei, blue)
Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis. BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation. The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.
Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. N.B. For long term storage the addition of 0.09% sodium azide is recommended.