|The BMI1 bmi1 (Catalog #MBS240598) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human BMI1 / PCGF4 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BMI1 / PCGF4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).
ELISA (1:5000 - 1:30000), ICC, IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the BMI1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BMI1 bmi1 product has the following accession number(s) (GI #27883842) (NCBI Accession #NP_005171.4) (Uniprot Accession #P35226). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human BMI1 / PCGF4 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-BMI-1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF) (Anti-Bmi1 Antibody - Immunofluorescence Microscopy. Immunofluorescence using affinity purified goat anti-Bmi1 shows nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3 M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4°C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).)
Western Blot (WB) (Anti-Bmi1 Antibody - Western Blot. Western blot of Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kD corresponding to human Bmi1 (arrowhead). Approximately 20 ug of an U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4C with the primary antibody diluted to 1:1000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Target Species: Human
Immunogen Description: Synthetic peptide from human BMI1.