anti-BLyS antibody product blog
Tags: Antibody; Monoclonal Antibody; BLyS; anti-BLyS antibody;
The BLyS tnfsf13b (Catalog #MBS200076) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BLyS antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s BLyS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the BLyS tnfsf13b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BLyS tnfsf13b product has the following accession number(s) (GI #5730097) (NCBI Accession #NP_006564) (Uniprot Accession #Q9Y275). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BLyS antibody with the following immunoassay(s):
Western Blot (WB) (Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))
B lymphocyte stimulator (BLyS),a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells.
Antigen Species: Human
Immunogen: Recombinant human BLyS (134-285aa) purified from E. coli. Clone Note: Anti-human BLyS mAb, clone H4-C7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BLyS protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BLyS are readily searchable from our website. Different antibodies against the same target such as BLyS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone Marrow, Brain, Kidney, Liver, Lung, Lymph Node, Skin, Thymus, Vascular tissues are correlated with this protein.