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anti-beta-Tubulin antibody product blog

Posted on 2014-02-02 16:00:52 by mybiosource_staff
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Tags: Antibody; beta-Tubulin; Monoclonal Antibody; anti-beta-Tubulin antibody;
The beta-Tubulin n/a (Catalog #MBS470004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-Tubulin reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunocytochemistry.
ELISA: 1:2000
Immunocytochemistry: 1:200
Western Blot: 1:1000. Researchers should empirically determine the suitability of the beta-Tubulin n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the beta-Tubulin with the following immunoassay(s):
Testing Data
Testing Data beta-Tubulin.

Testing Data 2
Testing Data 2 beta-Tubulin.

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/b-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in b-tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of bIII-tubulin in vitro. Unphosphorylated Ser-444 in bIII-tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in b-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

Immunogen: Clone (M154) was generated from purified porcine brain tubulin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing beta-Tubulin are readily searchable from our website. Different antibodies against the same target such as beta-Tubulin may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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