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anti-BAP31 antibody product blog

Posted on 2014-03-24 02:53:14 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; BAP31; anti-BAP31 antibody;
The BAP31 bcap31 (Catalog #MBS221316) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI BAP31 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s BAP31 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin, Western Blot (WB).
Western Blot: This item detects a band of approximately 28kDa in rat lung cell lysates.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1ug/ml. Researchers should empirically determine the suitability of the BAP31 bcap31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The BAP31 bcap31 product has the following accession number(s) (GI #213511012) (NCBI Accession #NP_001132913.1) (Uniprot Accession #P51572). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI BAP31 with the following immunoassay(s):
Testing Data #1 (Western blot analysis of rat lung lysate probed with Rabbit anti Human BAP31 (MBS221316) at 0.5(A), 1(B) and 2(C) ug/ml)
Testing Data #1 BAP31.

Testing Data #2 (Formilin fixed, paraffin embedded rat lung stained with Rabbit anti Human BAP31 (MBS221316) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Testing Data #2 BAP31.

MBS221316 recognises an epitope within the internal region of BAP31 (B cell receptor-associated protein 31), a 28kDa conserved type III integral membrane protein of the Endoplasmic Reticulum (ER), containing two caspase cleavage sites within its cytosolic domain. BAP31 appears to be a multi-functional ER-associated transport protein which was originally identified through its association with membrane IgD in B lymphocytes. Several proteins have since been shown to associate with BAP31, including the Beta2 integrin receptor CD11b/CD18, and nonmuscle gamma-actin and myosin B heavy chain. BAP31 has become a focus area for apoptosis studies due to its association with Bcl-2 and cleavage by initiator caspases including caspase 1 and 8. Cleavage of BAP31 generates a membrane-integrated p20 fragment which is itself a potent inducer of apoptosis when expressed ectopically.

Perservative Stabilisers: 0.02% Sodium Azide (NaN3). Immunogen: A 17 amino acid peptide located in the internal region of human BAP31.
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human BAP31 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BAP31 are readily searchable from our website. Different antibodies against the same target such as BAP31 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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