anti-BAD antibody product blog
Tags: Antibody; anti-BAD antibody; BAD; Polyclonal Antibody;
The BAD bad (Catalog #MBS151457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BAD Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s BAD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Bad antibody can be used for detection of Bad by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the BAD bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BAD bad product has the following accession number(s) (GI #17371773) (NCBI Accession #Q92934.3) (Uniprot Accession #Q92934). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BAD Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemical staining of rat thymus using Bad at 2 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 μg/mL.)
BAD Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.
Conjugate: Unconjugated
Immunogen: Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad.
Buffer: BAD Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BAD are readily searchable from our website. Different antibodies against the same target such as BAD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.