|The b-Catenin (pS37) ctnnb1 (Catalog #MBS462018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- beta-Catenin (S37) antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s b-Catenin (pS37) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the b-Catenin (pS37) ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The b-Catenin (pS37) ctnnb1 product has the following accession number(s) (GI #1125100) (Uniprot Accession #Q7ZU14). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti Phospho- beta-Catenin (S37) antibody with the following immunoassay(s):
Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the beta-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin (s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of beta-catenin targets the molecule for degradation via an ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
Antigen Preparation: A synthetic peptide corresponding to the epitope SGIHS with a single phosphorylation site Ser37 of human beta-catenin. Positive Control: HELA cell lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing b-Catenin (pS37) are readily searchable from our website. Different antibodies against the same target such as b-Catenin (pS37) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.