|The Axin1 axin1 (Catalog #MBS200185) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Axin1 antibody (AT1A4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Axin1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP).
The antibody has been tested by ELISA, Western blot, Immunofluorescence, and Immunoprecipitation analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the Axin1 axin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Axin1 axin1 product has the following accession number(s) (GI #227430349) (NCBI Accession #NP_001153070). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Axin1 antibody (AT1A4) with the following immunoassay(s):
Western Blot (WB) (Axin1 has 44% homology with Axin2 (Conductin) and its function is similar, but they have different expression patterns. Axin1 is widely expressed and detected at a very low level, whereas Axin2 is differentially expressed in various cells and tissue (stomach cancer or colorectal cancer). Also, it is reported that Axin2 was highly over-expressed in AGS and SW480 cell lines. Immunoprecipitation (IP) and western blot experiments were performed to evaluate Axin1 antibody\'s specificity. IP sample and cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-mouse Axin1 (1:500). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (Immunofluorescence of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500).)
Axin was originally identified as the product of the mouse gene called fused or fu (renamed Axin), and has since been shown to play a crucial role in controlling axis formation during embryonic development. Axin1 is characterized to be a tumor suppressor and numerous gene mutations have been identified throughout Axin1 from a number of different cancers. Axin can identify in a mouse mutant strain, created by random integration of a transgene. Also, Axin1 plays an important role in the formation of the embryonic neural axis. Recently, it was reported that Axin is found in mitotic spindles and centrosomes.
Antigen Species: Human
Clone: Anti-human Axin1 mAb, clone AT1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Axin1 protein.
Immunogen: Recombinant human Axin1 (546-752aa) purified from E Coli.