anti-AURKA antibody product blog
Tags: Antibody; anti-AURKA antibody; AURKA; Monoclonal Antibody; AURORA-A KINASE;
The AURKA aurka (Catalog #MBS212609) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI AURORA-A KINASE reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s AURORA-A KINASE can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB).Western Blot: This item detects a band of approximately 46kDa in human HeLa and mouse M-ICc12 cell lysates.
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the AURKA aurka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AURKA aurka product has the following accession number(s) (GI #38327562) (NCBI Accession #NP_003591.2) (Uniprot Accession #O14965). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI AURORA-A KINASE with the following immunoassay(s):
Testing Data (Recombinant Aurora kinase detected with Mouse anti Aurora-A kinase)
Mouse anri Human Aurora -A kinase monoclonal antibody, clone 35C1 recognizes human Aurora-A kinase also known as Aurora 2, breast tumor-amplified kinase and serine/threonine-protein kinase 6 or 15. Aurora kinase An is member of the Ser/Thr protein kinase family containing a single protein kinase domain, has a molecular weight of ~46kDa and is involved in mitotic spindle assembly (Ducat et al. 2004). Aurora-A kinase is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis (Katayama et al. 2003). Aurora A kinase appears to facilitate phosphorylation of centrin and co-localizes with it at centrosomes with maximum expression through prophase to late metaphase (Lukasiewicz et al. 2011). Mouse anti human Aurora-A kinase, clone 35C1 specifically recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity (Cremet et al. 2003).
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Histology Positive Control Tissue: Human 293 and mouse LLC1 cell lines.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AURKA are readily searchable from our website. Different antibodies against the same target such as AURKA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.