anti-ATG16L1 antibody product blog
Tags: Antibody; Polyclonal Antibody; ATG16; anti-ATG16L1 antibody; ATG16L1;
The ATG16L1 atg16l1 (Catalog #MBS150152) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG16 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ATG16 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).ATG16 antibody can be used for the detection of ATG16 by Western blot at 1 and 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 4 mug/mL. Researchers should empirically determine the suitability of the ATG16L1 atg16l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ATG16L1 atg16l1 product has the following accession number(s) (GI #124256480) (NCBI Accession #NP_110430) (Uniprot Accession #Q676U5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ATG16 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of ATG16 in Hela cells with ATG16 antibody at 4.75 μg/mL.)
ATG16 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
Conjugate: Unconjugated
Immunogen: ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human ATG16.
Buffer: ATG16 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ATG16L1 are readily searchable from our website. Different antibodies against the same target such as ATG16L1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.