anti-ATF3 antibody product blog
Tags: Antibody; ATF3; Monoclonal Antibody; anti-ATF3 antibody;
The ATF3 atf3 (Catalog #MBS120339) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human ATF3 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ATF3 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC).ICC: 2-100 ug/ml. Researchers should empirically determine the suitability of the ATF3 atf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ATF3 atf3 product has the following accession number(s) (GI #95102480) (NCBI Accession #NP_004015.4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse monoclonal antibody Anti-Human ATF3 with the following immunoassay(s):
Quality Control (Western blot analysis of immunized recombinant protein, using anti-ATF3 monoclonal antibody.)
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ATF3 mAb.)
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [NCBI Entrez Gene Summary].
Preparation: This antibody was purified using protein A column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Sterility: Filtered through a 0.22 um membrane.
Disposal: This antibody solution contains sodium azide (NaN3)as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produc an explosive compound. For safe disposal, the vial has to be washed thorughly with water.
Safety warning and precautions: Caution must be taken to avoid contact with skin or eyes. In such case, rinse thoroughly at once with water. Do not ingest, inhale or swallow. Seek medical attention inmediately. Wear appropiate protective clothing such as laboratory overalls, safety glasess and gloves. It is strongly advised that this product should be handled by people who have been well traied in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.