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anti-ARG1 antibody product blog

Posted on 2014-05-11 04:41:39 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; ARG1; liver Arginase; anti-ARG1 antibody;
The ARG1 arg1 (Catalog #MBS175738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-liver Arginase antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s liver Arginase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ARG1 arg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ARG1 arg1 product has the following accession number(s) (GI #114146) (NCBI Accession #P07824.2) (Uniprot Accession #P07824). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-liver Arginase antibody with the following immunoassay(s):
Western Blot (WB) (Anti-liver Arginase antibody, MBS175738, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate )
Western Blot (WB) ARG1.

Description: Rabbit IgG polyclonal antibody for Arginase-1(ARG1) detection. Tested with WB in Mouse, Rat.
Background: ARG1(arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al.(1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat liver Arginase (307-323aa KREGNHKPETDYLKPPK), different from the related mouse sequence by two amino acids. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ARG1 are readily searchable from our website. Different antibodies against the same target such as ARG1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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