anti-AQP4 antibody product blog
Tags: Antibody; Aquaporin 4; Polyclonal Antibody; anti-AQP4 antibody; AQP4;
The AQP4 aqp4 (Catalog #MBS175277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Aquaporin 4 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Aquaporin 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the AQP4 aqp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The AQP4 aqp4 product has the following accession number(s) (GI #2506859) (NCBI Accession #P55087.2) (Uniprot Accession #P55087). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Aquaporin 4 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-Aquaporin 4 antibody, MBS175277, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: COLO320 Cell Lysate
)
Description: Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB in Human, Mouse, Rat.
Background: Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1.Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence(designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (172-188aa FTIFASCDSKRTDVTGS), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AQP4 are readily searchable from our website. Different antibodies against the same target such as AQP4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.