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anti-APSE-1_38 antibody product blog

Posted on 2013-12-19 12:12:57 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; p38; anti-APSE-1_38 antibody; APSE-1_38;
The APSE-1_38 n/a (Catalog #MBS194397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s p38 can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunoprecipitation.
Immunoblotting: use at 1:5,000 dilution. A band of ~43 kDa is detected.
Immunoprecipitation: use at 1:250 dilution.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate. Researchers should empirically determine the suitability of the APSE-1_38 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The APSE-1_38 n/a product has the following accession number(s) (GI #9633585) (NCBI Accession #NP_050999.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

The MAPK (mitogen-activated protein kinase) family includes stress-activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases. Upon activation, p38 translocates to the cell nucleus where it phosphorylates one or more nuclear substrates resulting in transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase-3 and its downstream effector PARP. p38 is activated by chemical stress inducers (such as hydrogen peroxide and heavy metals) and biological stress signals (such as tumor necrosis factor, interleukin-1, irradiation, and chemotherapeutic drugs).

APSE-1_38 n/a

Antigen: A 20 amino acid synthetic peptide (aa 341-360) corresponding to human p38. Preservatives: None.
Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing APSE-1_38 are readily searchable from our website. Different antibodies against the same target such as APSE-1_38 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.
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