anti-APP antibody product blog
Tags: Antibody; Polyclonal Antibody; APP; anti-APP antibody;
The APP app (Catalog #MBS150418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s APP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).APP antibody can be used for detection of APP and Ab peptide by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. Researchers should empirically determine the suitability of the APP app for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The APP app product has the following accession number(s) (GI #28721) (NCBI Accession #CAA30050) (Uniprot Accession #P05067). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the APP Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of APP in (A) human, (B) mouse, and (C) rat brain tissue lysates with APP antibody at 1 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of APP in human brain (Alzheimer�s disease) tissue with APP antibody at 10 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of APP in human brain tissue with APP antibody at 2.5 μg/ml.)
Immunofluorescence (IF) (Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 μg/mL.
Green: APP Antibody (2133)
Blue: DAPI staining)
APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer\'s disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer\'s disease.
Conjugate: Unconjugated
Immunogen: APP antibody was raised against a peptide corresponding to 10 amino acids of human amyloid A4 protein precursor (APP) corresponding to the amino terminus of the 4K Ab peptide generated by beta- and gamma-secretases.
Buffer: APP Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing APP are readily searchable from our website. Different antibodies against the same target such as APP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.