anti-ApoE antibody product blog
Tags: Antibody; APOE; Monoclonal Antibody; anti-APOE antibody;
The ApoE apoe (Catalog #MBS850083) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ApoE Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ApoE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the ApoE apoe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ApoE apoe product has the following accession number(s) (GI #4557325) (NCBI Accession #NP_000032.1) (Uniprot Accession #P02649). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ApoE Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using ApoE mAb against HEK293 (1) and ApoE-hIgGFc transfected HEK293 (2) cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining.)
Testing Data
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Immunogen: Purified recombinant fragment of human ApoE expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ApoE are readily searchable from our website. Different antibodies against the same target such as ApoE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Alzheimer Disease, Arteriosclerosis, Atherosclerosis, Cardiovascular Diseases, Cognition Disorders, Coronary Disease, Hypercholesterolemia, Hyperlipidemias, Inflammation, Plaque, Atherosclerotic are some of the diseases may be linked to ApoE Antibody. Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Nerve, Spleen, Vascular tissues are correlated with this protein.