|The APOA1 apoa1 (Catalog #MBS200214) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The APOA1 antibody (clone AT1E12) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s APOA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500~1:5000. Recommended staring dilution is 1:1000. Researchers should empirically determine the suitability of the APOA1 apoa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The APOA1 apoa1 product has the following accession number(s) (GI #4557321) (NCBI Accession #NP_000030.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the APOA1 antibody (clone AT1E12) with the following immunoassay(s):
Western Blot (WB) (The extracts of HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Apolipoprotein A1 (APOA1) is the main protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in APOA1 are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. High level of APOA1 is regarded as protective against atherosclerosis and cardiovascular disease.
Antigen Species: Human
Clone: Anti-human APOA1 mAb, clone AT1E12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human APOA1 protein.
Immunogen: Recombinant human APOA1 (25-267aa) purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing APOA1 are readily searchable from our website. Different antibodies against the same target such as APOA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.