anti-APEX1 antibody product blog
Tags: Antibody; APEX1; anti-APEX1 antibody; Polyclonal Antibody; APE1;
The APEX1 apex1 (Catalog #MBS175622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-APE1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s APE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the APEX1 apex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The APEX1 apex1 product has the following accession number(s) (GI #113984) (NCBI Accession #P27695.2) (Uniprot Accession #P27695). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-APE1 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-APE1 antibody, MBS175622, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
)
Immunohistochemistry (IHC) (Anti-APE1 antibody, MBS175622, IHC(P)
IHC(P): Rat Intestine Tissue )
Western Blot (WB) (Anti-APE1 antibody, MBS175622, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HELA Cell Lysate
)
Description: Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: APEX1, also called apurinic endonuclease(APE), is a DNA repair enzyme having apurinic/apyrimidinic(AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human APE1 (177-191aa RGLVRLEYRQRWDEA), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing APEX1 are readily searchable from our website. Different antibodies against the same target such as APEX1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.