anti-APAF1 antibody product blog
Tags: Antibody; APAF1; Polyclonal Antibody; anti-APAF1 antibody;
The APAF1 apaf1 (Catalog #MBS151391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Apaf1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Apaf1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 mug/mL. Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 20 mug/mL. Researchers should empirically determine the suitability of the APAF1 apaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The APAF1 apaf1 product has the following accession number(s) (GI #2330015) (NCBI Accession #AAC51678) (Uniprot Accession #O14727). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Apaf1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 μg/mL dilution in the absence (A) or presence (B) of blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 μg/mL.)
Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
Conjugate: Unconjugated
Immunogen: Apaf1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.
Buffer: Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing APAF1 are readily searchable from our website. Different antibodies against the same target such as APAF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.