anti-anti-DYKDDDDDK antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-DYKDDDDDK; anti-anti-DYKDDDDDK antibody;
The anti-DYKDDDDDK n/a (Catalog #MBS560058) is an Antibody and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Chickens were immunized with the DYKDDDDK peptide conjugated to a protein carrier. Eggs were collected and an IgY fraction was prepared. The resulting fraction was passed over an antigen containing immunoabsorbant from which the anti-DYKDDDDK was immunoaffinity purified. Purification was followed by conjugation with fluorescein isothiocyanate (FITC).
Buffer: Phosphate buffered saline, pH 7.2 with 0.2% BSA. Preservative: 0.09% sodium azide
Procedure: Chickens were immunized with DYKDDDDK peptide conjugated with KLH. Eggs were collected and the Ig fraction prepared. Antibodies were immunoaffinity purified using the peptideimmobilized on a solid phase matrix then conjugated with fluorescein isothiocyanate (FITC). Antibodies concentration was determined using an absorbance at 280 nm: 1.32 equals 1.0 mg of IgY. FITC/Protein Ratio = 3.0-6.0. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing anti-DYKDDDDDK are readily searchable from our website. Different antibodies against the same target such as anti-DYKDDDDDK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.