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anti-AMPK-alpha (pT172) antibody product blog

Posted on 2014-09-01 21:07:55 by mybiosource_staff
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Tags: Antibody; AMPK-alpha (pT172); anti-AMPK-alpha (pT172) antibody;
The AMPK-alpha (pT172) n/a (Catalog #MBS462009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- AMPK alpha 1 (pThr 172) reacts with Human, Rat, Mouse, Bovine, Canine, Chicken and may cross-react with other species as described in the data sheet. MyBioSource\'s AMPK-alpha (pT172) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the AMPK-alpha (pT172) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The AMPK-alpha (pT172) n/a product has the following accession number(s) (GI #60459958). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti Phospho- AMPK alpha 1 (pThr 172) with the following immunoassay(s):
Testing Data
Testing Data AMPK-alpha (pT172).

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2).

Antigen Preparation: A synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha 1 protein from human origin. It is identical among human, mouse, rat, chicken, bovine, dog, and insect species. Positive Control: Serum starved HEK293
Cellular Location: Cytoplasmic. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AMPK-alpha (pT172) are readily searchable from our website. Different antibodies against the same target such as AMPK-alpha (pT172) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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