|The AKT1 akt1 (Catalog #MBS631826) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Akt1, phosphorylated (Ser473) (Rac PKa, PKBa) reacts with Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Akt1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Immunoblot Analysis: 0.5-1ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100ng/ml PDGF for 20 minutes. Non-treated (lane 1) or PDGF-treated (lane 2) mouse NIH-3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-Akt1/PKBa (Ser473) (0.5ug/ml). Researchers should empirically determine the suitability of the AKT1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AKT1 akt1 product has the following accession number(s) (GI #18027298) (Uniprot Accession #P31749). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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The serine/threonine kinase Akt1 has also been described as Rac-PKa or PKBa. Rac protein kinase family members feature a pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein beta gamma subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. Akt1 is a protein of 480 amino acids and has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
Immunogen: KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AKT1 are readily searchable from our website. Different antibodies against the same target such as AKT1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. AKT1 also interacts with the following gene(s): AKT2, BAD, CASP3, CASP9, FOXO1, IRS1, MTOR, NOS3, PTEN, SRC.