anti-Akt1 antibody product blog
Tags: Antibody; anti-AKT1 antibody; AKT1; Monoclonal Antibody; Akt, pan;
The Akt1 akt1 (Catalog #MBS604688) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Akt, pan can be used in a range of immunoassay formats including, but not limited to, Suitable for use in Immunoprecipitation.Other applications not tested.
Recommended Dilutions:
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Akt1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Akt1 akt1 product has the following accession number(s) (GI #24647360) (NCBI Accession #NP_732115.1) (Uniprot Accession #Q8INB9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Akt, pan (Rac PKa, PKBa) (Agarose) with the following immunoassay(s):
Immunoprecipitation (IP) (Immunoprecipitation of Jurkat cell lysates using Immobilized Mouse IgG (Bead Conjugate) (lane 1) and Immobilized MBS604688 (Bead Conjugate) (lane 2). The western blot was probed using MBS604688.)
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip and p21 Waf1/CIP1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex. Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation.