|The Akt (Thr-34) n/a (Catalog #MBS472002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Akt (Thr-34), phospho-specific reacts with Human, Rat, Rabbit and may cross-react with other species as described in the data sheet. MyBioSource\'s Akt (Thr-34) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot.
Western Blot: 1:1000. Researchers should empirically determine the suitability of the Akt (Thr-34) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Akt (Thr-34), phospho-specific with the following immunoassay(s):
Akt, also known as Protein Kinase B (PKB) and Rac (Related to the An and C kinases), is a 60kDa serine/threonine kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4- bisphosphate which may induce the membrane translocation of Akt coincident with its phosphorylation and activation. Upon activation, Akt associates with members of the PKC family of kinases, such as PKC-delta and PKC-zeta. Ceramide-activated PKC-zeta leads to phosphorylation of the Akt-Pleckstrin Homology (PH) domain on threonine 34. This phosphorylation inhibits PIP3 binding to Akt, preventing activation of the kinase by insulin. Since Akt confers a prosurvival signal and regulates pathways in response to insulin, suppressing its activation may be one mechanism by which ceramide promotes cell death and induces insulin resistance.
Immunogen: Phospho-Akt (Thr-34) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 34 of human Akt. This sequence is highly conserved in rat and mouse Akt. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Akt (Thr-34) are readily searchable from our website. Different antibodies against the same target such as Akt (Thr-34) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.