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anti-AKT antibody product blog

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Posted on 2014-09-05 05:52:01 by mybiosource_staff
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Tags: Antibody; anti-Akt antibody; Akt; Polyclonal Antibody;
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The AKT akt (Catalog #MBS440053) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKT (C20) Antibody reacts with Mouse, rat, human and may cross-react with other species as described in the data sheet. MyBioSource\'s AKT can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the AKT akt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The AKT akt product has the following accession number(s) (GI #51539256) (NCBI Accession #AAU06122.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Akt1, also known as Protein kinase B, is a serine/threonine kinase that is at the center of many signal transduction pathways. Akt1 regulates glucose metabolism, transcription, apoptosis, cell proliferation, angiogenesis, and cell motility (1). The activation of Akt1 occurs downstream of PI-3K, which upon activation by an extracellular molecule, generates PIP (3) (2). PIP (3) is a lipid second messenger that promotes the translocation of Akt1 to the plasma membrane via PH (pleckstrin-homology) domain binding (2-4). At the plasma membrane Akt1 is phosphorylated and activated by PDK-1 at its catalytic site on residue Thr308 (1). Residue Ser473 is also phosphorylated at this time but the kinase has not yet been identified (1). In this activated form Akt1 phosphorylates proteins that contain the motif, R-x-R-x-x-S/T-F/L, at the plasma membrane, in the cytosol, and in the nucleus (1). In addition to its kinase activity, Akt1 forms complexes with other proteins that are not substrates. These other proteins act as modulators of Akt1 activity (1).

AKT akt

Origin: Akt1 (C20) is provided as an affinity purified rabbit polyclonal antibody, raised against a synthetic peptide mapping to the carboxy terminal domain of human Akt1. Immunogen: Synthetic peptide mapping to the carboxy terminal domain of human AKT1. Differs from corresponding mouse sequence by one residue. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AKT are readily searchable from our website. Different antibodies against the same target such as AKT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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