|The AK3 ak3 (Catalog #MBS200020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AK3 antibody (clone SJB3-36) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s AK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Recombinant protein,Cell lysate), Immunohistochemistry (IHC).
The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the AK3 ak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AK3 ak3 product has the following accession number(s) (GI #19923437) (NCBI Accession #NP_057366). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AK3 antibody (clone SJB3-36) with the following immunoassay(s):
Immunohistochemistry (IHC) (Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.)
Western Blot (WB) (The recombinant human Ak isozymes (Ak1, Ak2, Ak3) and mouse liver were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Ak3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 220.127.116.11) is an ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.
Antigen Species: Human
Clone: Anti-human Ak3 mAb, clone SJB3-36, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Ak3 protein.
Immunogen: Recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AK3 are readily searchable from our website. Different antibodies against the same target such as AK3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.