Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow anti-AK3 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • Mescaline (HRP) hrp conjugate blog
 • ca2 biological reagent blog
 • CDHR3 sirna blog
 • Non-Prostatic Acid Phosphatase protein blog
 • TMEM106B sirna blog
 • ANKRD7 sirna blog
 • anti-PRKCE antibody blog
 • USP44 sirna blog
 • RCAN3 blocking peptide blog
 • anti-NMDA1 antibody blog
 • anti-hpdl antibody blog
 • anti-S100 antibody blog
 • E-I-L-D-V peptide blog
 • SEC14L2 blocking peptide blog
 • IgG secondary antibody blog
 • HSPA12B sirna blog
 • AA elisa kit blog
 • HIST1H1E sirna blog
 • beta2-GP1 Ab IgM elisa kit blog
 • SDF 1a recombinant protein blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Caspase 14 (CASP14) ELISA Kit
 • gp120-160 (SIV-mac239) Antibody
 • growth hormone releasing hormone receptor (GHRHR) ELISA Kit
 • Apolipoprotein B-100 (APOB) Antibody
 • Insulin receptor-related protein (INSRR) Recombinant Protein
 • WDR76 cDNA Clone
 • ZIC5 Peptide
 • Collagen alpha-1(XVIII) chain (COL18A1) Recombinant Protein
 • Filamin A, Alpha (FLNa) ELISA Kit
 • CD8 ALPHA Antibody
 • Sperm (AsAb IgG) ELISA Kit
 • Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNP A1) ELISA Kit
 • MAPK1 Antibody
 • CX43 ELISA Kit
 downarrow more ...
separator

anti-AK3 antibody product blog

separator
Posted on 2015-04-22 14:46:56 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Monoclonal Antibody; AK3; anti-AK3 antibody;
separator
The AK3 ak3 (Catalog #MBS200020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AK3 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s AK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).
WB: 1:500 ~ 1:2,000.
Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the AK3 ak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The AK3 ak3 product has the following accession number(s) (GI #19923437) (NCBI Accession #NP_057366) (Uniprot Accession #Q9UIJ7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AK3 antibody with the following immunoassay(s):
Western Blot (WB) (The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AK1 Lane 2. : Recombinant Human AK2 Lane 3. : Recombinant Human AK3 The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : Mouse kidney tissue lysate The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse liver tissue lysate)
Western Blot (WB) AK3.

Immunohistochemistry (IHC) (Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.)
Immunohistochemistry (IHC) AK3.

Immunofluorescence (IF) (ICC/IF analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF) AK3.

Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is an ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions