anti-Aggrecan G1-IGD-G2 Domains antibody product blog
Tags: Antibody; Monoclonal Antibody; Aggrecan G1-IGD-G2 Domains; anti-Aggrecan G1-IGD-G2 Domains antibody;
The Aggrecan G1-IGD-G2 Domains n/a (Catalog #MBS608516) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Aggrecan G1-IGD-G2 Domains (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, M reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Aggrecan G1-IGD-G2 Domains can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.
Dilution: Direct ELISA: 0.5-1.0ug/ml the detection limit is ~2ng/well
Western Blot: 1-2ug/ml the detection limit is ~5ng/lane under non-reducing conditions
Immunohistochemistry: 25ug/ml detects aggrecan in paraffin-embedded human cartilage tissue sections.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human aggrecan from conditioned media of transfected NSO cells. Dectection using A1059-53H. Researchers should empirically determine the suitability of the Aggrecan G1-IGD-G2 Domains n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases. The deduced amino acid sequence of human aggrecan core protein consists of 2415 residues and predicts a signal peptide and domains of G1, IGD, G2, KS, CS1, CS2 and G3 (3). Two globular domains, G1 and G2, comprise the Nterminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the Cterminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250kD core protein, aggrecan monomers exist as a 1000-2000kD molecule.
Immunogen: Recombinant corresponding to aa20-675 from human Aggrecan G1-IGD-G2 Domains, expressed in NS0 cells (NP_037359). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Aggrecan G1-IGD-G2 Domains are readily searchable from our website. Different antibodies against the same target such as Aggrecan G1-IGD-G2 Domains may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.