anti-AFP antibody product blog
Tags: Antibody; AFP; Polyclonal Antibody; anti-AFP antibody; a-Fetoprotein;
The AFP afp (Catalog #MBS6004901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The a-Fetoprotein, NT (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein Precursor, FETA, HPAFP) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s a-Fetoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AFP afp product has the following accession number(s) (GI #4501989) (NCBI Accession #NP_001125.1) (Uniprot Accession #P02771). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the a-Fetoprotein, NT (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein Precursor, FETA, HPAFP) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of AFP Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AFP (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. This protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Immunogen: Synthetic peptide selected from the N-terminal region of human AFP (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AFP are readily searchable from our website. Different antibodies against the same target such as AFP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.